Optimisation of the lymphocyte proliferation assay in rainbow trout (Oncorhynchus mykiss)
نویسندگان
چکیده
منابع مشابه
Rainbow Trout, Oncorhynchus mykiss
Rainbow trout are classified as Oncorhynchus mykiss, and as such belong to the same genus as Pacific salmon, and to the family Salmonidae, which includes Atlantic salmon (Salmo salar), various trout (Salvelinus sp.), Arctic char (Salvelinus alpinus), Arctic grayling (Thymallus arcticus) and whitefish (Coregonus sp.). Rainbow trout are native to areas around the North Pacific Ocean, from souther...
متن کاملMetabolism in Erythrocytes of the Rainbow Trout Oncorhynchus Mykiss
Experiments were carried out to investigate the relationship between ion exchange and energy metabolism in rainbow trout erythrocytes in vitro. Under resting conditions, the sodium/potassium pump accounts for 20 % of the cellular energy budget. In the presence of the /S-adrenergic agonist isoproterenol, however, this increases to 43 %. Inhibition of the sodium/potassium pump with ouabain result...
متن کاملVitellogenin receptors during vitellogenesis in the rainbow trout Oncorhynchus mykiss.
Rainbow trout vitellogenin receptors have been characterized by ligand blotting and Scatchard analysis. Their evolution has been studied over a reproductive cycle in a broodstock of 2-year-old females. The receptors were prepared from ovarian membrane homogenates and were solubilized using n-octyl-beta-D-glucopyranoside. The visualization of the receptor by ligand blotting using 125iodine-vitel...
متن کاملThe Effects of Branchial Chloride Cell Proliferation on Respiratory Function in the Rainbow Trout Oncorhynchus Mykiss
The objectives of this study were to induce chloride cell (CC) proliferation on the gill lamellae of rainbow trout Oncorhynchus mykiss and to evaluate the consequences for respiratory function. Chronic elevation of hormone levels was used to induce CC proliferation; fish were injected with a combination of cortisol (8 mg kg-1 intramuscularly every day for 10 days) and ovine growth hormone (2 mg...
متن کاملGenetic diversity in Chilean populations of rainbow trout, Oncorhynchus mykiss
The rainbow trout Oncorhynchus mykiss, was first introduced in Chile between 1905 and 1920 and is currently widely distributed in Chile from Antofagasta (23°S) to Patagonia (55oS). The broad range of the geographic and climatic distributions of this species in Chile offers a unique opportunity to study the effect of naturalization of an introduced species on its genetic variability. It is of pa...
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ژورنال
عنوان ژورنال: Veterinární Medicína
سال: 2019
ISSN: 0375-8427,1805-9392
DOI: 10.17221/98/2019-vetmed